In vitro digestion of fish oils rich in n-3 polyunsaturated fatty acids studied in emulsion and at the oil–water interface
Résumé
At the junction of chemistry, physics and biology, digestion involves many processes.
Studying the mechanisms in such a complex system is challenging because numerous
interactions coexist. Even in an apparently simple system such as emulsion, many
physicochemical characteristics affect lipid digestion. Moreover, these characteristics are
difficult to control using conventional in vitro techniques. The goal of this work was to design
a microfluidic device allowing the study of well-controlled individual oil droplets in
gastrointestinal digestion conditions. Different parameters were investigated in order to
validate the relevance of this device compared to conventional in vitro techniques using
emulsions. Various triglycerides and digestion conditions were tested with droplets of the
same initial diameter generated by a flow focusing device, then placed in individual traps of a
microfluidic chamber for digestion with continuous digestive juice renewal. The results are in
good agreement with those obtained with conventional in vitro techniques and open the way
to screening of lipid digestion, in particular of lipophilic molecules bioaccessibility, a
prerequisite for bioavailability studied in nutrition, pharmacology, and toxicology.
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