Beyond amylose content, selecting starch traits impacting in vitro α-amylase degradability in a wheat MAGIC population - Archive ouverte HAL Access content directly
Journal Articles Carbohydrate Polymers Year : 2022

Beyond amylose content, selecting starch traits impacting in vitro α-amylase degradability in a wheat MAGIC population

(1) , (1) , (2) , (2) , (1) , (3)
1
2
3

Abstract

A major challenge faced when studying the "structure-degradability" interaction of native starch is deciphering the interdependency between different structural levels, especially when experimental conditions limit the number of samples. To tackle this challenge, 224 wheat starches from a 4-way multiparent advanced generation inter-cross population were screened for structural features and degradation profiles by porcine pancreatic alpha-amylase. A hierarchical clustering on principal components (HCPC) were used as multifactorial analysis to explore the da; ta structure. The degradation procedure was proved to be robust and sensible enough to screen a large collection of starches. The HCPC highlighted the combined effects of granule size distribution (GSD), amylopectin chain length distribution (CLD), amylose content and endogenous alpha-amylase activity on degradation kinetics. Especially the GSD and amylopectin CLD showed high co-occurrences with specific hydrolysis profiles. These findings provide an innovative screening method and structural factors to be primarily considered for wheat starch selection in breeding programs.

Dates and versions

hal-03749046 , version 1 (10-08-2022)

Identifiers

Cite

Yuzi Wang, Kamal Kansou, Jenifer Pritchard, Alexander Zwart, Luc Saulnier, et al.. Beyond amylose content, selecting starch traits impacting in vitro α-amylase degradability in a wheat MAGIC population. Carbohydrate Polymers, 2022, 291, pp.119652. ⟨10.1016/j.carbpol.2022.119652⟩. ⟨hal-03749046⟩

Collections

INRAE
13 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More