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Journal Articles Talanta Year : 2022

Development of a multi-hormone analysis method by LC-MS/MS for environmental water application using diffusive gradient in thin films

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Rachel Martins de Barros
  • Function : Author
Sophie Lissalde
  • Function : Author
Robin Guibal
  • Function : Author
Gilles Guibaud
  • Function : Author

Abstract

An analysis method for four families of hormones (estrogens, progestins, androgens and prostaglandins), dedicated to an efficient water monitoring with passive sampling, was developed using a liquid chromatography tandem mass spectrometry with triple quadrupole coupling and universal electrospray ionisation. Thirteen natural and synthetic hormones in ultra-pure water could be analysed in a single run according to the French Standard NF T90-210: calibration range of 0.1 (except for 17 beta-Estradiol, Estriol, Estrone and Diethylstilbestrol, from 0.5 mu g/L; and Ethinylestradiol, from 1 mu g/L) to 20 mu g/L with linear regressions (R2 >= 0.96), maximum accuracy deviations of 30% at intermediate fidelity for three concentration references (1, 10 and 20 mu g/L) and instrumental LOQs from 0.05 to 1 mu g/L. The stability of 11 hormones (10 mu g/L) was studied under several storage conditions and sample evaporation. All selected hormones were stable for 60 days at -18 degrees C, 7 days at 4 degrees C and 7 days at 20 degrees C but continued drying flow after evaporation should be avoided, especially for 17 alpha-Estradiol, Estrone and Diethylstilbestrol. Observed matrix effects using o-DGT extracts (diffusive gradient in thin-film sampler for polar organics) containing an environmental matrix varied from 24 to 92% but all matrix effects were corrected with IS use. Therefore, the developed method, coupled with o-DGT, was tested with the o-DGT deployment in rivers. Using diffusion coefficients from the literature or lab determined, the concentrations in the rivers varied for Estrone from 1.8 ng/L to 2.5 ng/L, and for Androstenedione from 0.4 to 1.1 ng/L.
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Dates and versions

hal-03837850 , version 1 (03-11-2022)

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Rachel Martins de Barros, Sophie Lissalde, Robin Guibal, Gilles Guibaud. Development of a multi-hormone analysis method by LC-MS/MS for environmental water application using diffusive gradient in thin films. Talanta, 2022, 243, pp.123390. ⟨10.1016/j.talanta.2022.123390⟩. ⟨hal-03837850⟩
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