Variable and core functional microbiota associated with seven Medicago truncatula genotypes - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Conference Papers Year : 2023

Variable and core functional microbiota associated with seven Medicago truncatula genotypes

Zhu, Liya
  • Function : Author
  • PersonId : 1223411
Olivier Rué
Florence Deau
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  • PersonId : 1206841
Julie Cremaschi
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  • PersonId : 1113268
Valentin Loux
Samuel Mondy
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  • PersonId : 749176
  • IdHAL : samuelmondy
Jingjing Peng
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Sylvie Mazurier
Philippe Lemanceau
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  • PersonId : 1147674
Barbara Pivato


The rhizosphere microbiota emerges as a novel component that expands plant adaptation to its environment, and thus paves the way for future breeding approaches. Knowledge regarding the "functional core microbiota", which refers to the microbial taxa bearing essential functions for holobiont fitness, is required to move forward in this area. The aim of the present study was to compare the functional microbiota recruited by different plant genotypes in order to identify its core and variable components. Seven genotypes of Medicago truncatula (DZA315-16, DZA315-26, DZA045-6, F83005-5, SA028064, Jemalong A17 and J6), representatives of a core collection of 184 genotypes, were studied. Functional genes have been described using shotgun metagenomics followed by Prodigal. Core and variable functional microbiota have been identified based on shared genes and on genes explaining differences among the genotypes, respectively. Functional assignments have been obtained based on proteins annotation and Gene Onthology using BlastX and EggNOG. 76,264 genes were shared, whilst 3547 genes explained the differences between the three DZA genotypes and SA, 4662 among the three DZA and the F, A17, J6 genotypes, and 32 among SA and the three genotypes F, A17, J6. 60.5% of these genes have been assigned to biological processes, 22.6% to cellular components and 16.9% to molecular functions. Particularly, inorganic ion, amino-acid, and carbohydrate transport were the most represented in biological processes. This global analysis shows that different genotypes modulate rhizosphere microbial functional genes and provides new insights into the variable and core microbiota among different plant genotypes
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Dates and versions

hal-04139303 , version 1 (23-06-2023)


  • HAL Id : hal-04139303 , version 1


Zhu, Liya, Olivier Rué, Florence Deau, Julie Cremaschi, Valentin Loux, et al.. Variable and core functional microbiota associated with seven Medicago truncatula genotypes. 12th International PGPR Workshop 2023, May 2023, Toulouse, France. ⟨hal-04139303⟩
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