Despite the extensive use of sampling to estimate the average number of grape bunches per vine, there is no clearly established sampling protocol that can be used as a reference when performing these estimations. Each practitioner therefore has their own sampling protocol. This study characterised the effect of differences between sampling protocols in terms of estimation errors. The goal was to identify the most efficient practices that will improve the early estimation of an important yield component: average bunch number. First, the appropriateness of including non-productive vines (i.e., dead and missing vines) in the sampling protocol was tested; the objective was to determine whether it is relevant to estimate two yield components simultaneously. Second, sampling protocols with sampling sites of varying size were compared to determine how the spatial distribution of observations and potential spatial autocorrelation affect estimation error. Third, a new confidence interval for estimation error was determined to express expected error as a percentage. It aimed at designing a new tool for finding the best sample size in an operational context. Tests were performed on two vineyards in the South of France, in which the number of bunches per vine had been exhaustively determined on all the plants before flowering. The results show that the simultaneous estimation of number of bunches and proportion of dead and missing vines increased the estimation errors by a factor of 2. Despite the low spatial autocorrelation of bunch number, the results show that the observation must be spread across at least 2 or 3 sampling sites to reduce estimation errors. Finally, the confidence intervals expressed as a percentage were validated and used to define an adequate sample size based on a compromise between the expected precision and the variability observed in the first measurements.