Riparian forests are considered as complex and biologically rich habitats. They play a crucial role for biodiversity conservation, in particular in landscapes under strong management pressure. Tree-related microhabitats (TreMs) support a wide array of biodiversity and some authors have suggested using them as indirect biodiversity indicators in forest ecosystems. This study investigates TreM profiles of a temperate riparian forest as last natural forest ecosystem in a context of intensively managed landscape along the Ciron river and of surrounding pine plantations of the Landes de Gascogne forest (France) and explores their drivers at tree and plot scales. A total of 28 transects perpendicular to the river, representing 84 0.1 ha-plots, were selected along the Ciron river and three of its tributaries. At tree scale, diameter at breast height (dbh), tree species and tree status (living, standing or lying dead) were significant predictors of TreM diversity. At plot scale, Alnus glutinosa was found to significantly contribute to riparian forest TreM profiles by hosting specific TreMs. The same role was highlighted for broadleaved species in pine plantations. At plot scale, the drivers of TreM diversity varied depending on plot location in the transect (riparian forest, plantation forest, intermediate area). We found that the presence of lying dead trees is an important driver of TreM diversity at plot scale, particularly in plantation stand and we thus suggest lying dead trees be included in future studies focusing on TreMs. The rich TreM profile of riparian forests highlights their crucial role in biodiversity conservation.