CAM evolution is associated with gene family expansion in an explosive bromeliad radiation - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Preprints, Working Papers, ... Year : 2023

CAM evolution is associated with gene family expansion in an explosive bromeliad radiation

Clara Groot Crego
Jaqueline Hess
Gil Yardeni
Marylaure de la Harpe
Clara Priemer
  • Function : Author
Francesca Beclin
  • Function : Author
Sarah Saadain
  • Function : Author
Luiz Cauz-Santos
Eva Temsch
Hanna Weiss-Schneeweiss
Michael H.J. Barfuss
Walter Till
  • Function : Author
Wolfram Weckwerth
  • Function : Author
Karolina Heyduk
  • Function : Author
Christian Lexer
Ovidiu Paun

Abstract

1. Abstract The subgenus Tillandsia (Bromeliaceae) belongs to one of the fastest radiating clades in the plant kingdom and is characterised by the repeated evolution of Crassulacean Acid Metabolism (CAM). Despite its complex genetic basis, this water-conserving trait has evolved independently across many plant families and is regarded as a key innovation trait and driver of ecological diversification in Bromeliaceae. By producing high quality genome assemblies of a Tillandsia species pair displaying divergent photosynthetic phenotypes, and combining genome-wide investigations of synteny, TE dynamics, sequence evolution, gene family evolution and temporal differential expression, we were able to pinpoint the genomic drivers of CAM evolution in Tillandsia . Several large-scale rearrangements associated with karyotype changes between the two genomes and a highly dynamic TE landscape shaped the genomes of Tillandsia . However, our analyses show that rewiring of photosynthetic metabolism is mainly obtained through regulatory evolution rather than coding sequence evolution, as CAM-related genes are differentially expressed across a 24-hour cycle between the two species, but are no candidates of positive selection. Gene orthology analyses reveal that CAM-related gene families manifesting differential expression underwent accelerated gene family expansion in the constitutive CAM species, further supporting the view of gene family evolution as a driver of CAM evolution.

Dates and versions

hal-04338234 , version 1 (12-12-2023)

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Clara Groot Crego, Jaqueline Hess, Gil Yardeni, Marylaure de la Harpe, Clara Priemer, et al.. CAM evolution is associated with gene family expansion in an explosive bromeliad radiation. 2023. ⟨hal-04338234⟩
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