Laser Capture Microdissection to study iron homeostasis gene expression in Bacillus cereus during Galleria mellonella midgut infection - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Conference Papers Year : 2018

Laser Capture Microdissection to study iron homeostasis gene expression in Bacillus cereus during Galleria mellonella midgut infection

Abstract

Iron acquisition is essential for pathogenic bacteria in DNA synthesis, enzyme activities, and respiration. However, free iron is toxic for organisms: it is bound to other molecules like hemoproteins, transferrin or stored in ferritin. To overcome this lack of free iron, bacteria possess several systems to acquire bound iron, by surface proteins or secreted iron chelating siderophores. In the gut iron homeostasis is complex since host cells, commensal microbiota and food-iron are interacting to maintain this balance and little is known about genes involved during gut infection. Our work addresses this purpose in Bacillus cereus, (closely related to the insect pathogen Bacillus thuringiensis). The presentation focus on genes related to iron homeostasis expressed during gut infection in the larvae of the insect model, Galleria mellonella (Greater wax moth). To perform these analyses, we adapted Laser Capture Microdissection to collect B. cereus cells in cryo-sections of previously force fed germ-free larvae and assessed targeted gene expression by RT-qPCR from the recovered mRNA. The results showed modulation of several iron homeostasis related genes at 16h (bacteria colonising the intestine surface) compared to 3h (bacteria in the lumen) post infection. Further analyzes are ongoing to characterize physiological and chemical markers in the gut environment.
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Dates and versions

hal-04370041 , version 1 (02-01-2024)

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  • HAL Id : hal-04370041 , version 1

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Christina Nielsen-Leroux, Laurent Consentino, Agnès Réjasse, Nicolas Crapart, Claudia Bevilacqua. Laser Capture Microdissection to study iron homeostasis gene expression in Bacillus cereus during Galleria mellonella midgut infection. The 2018 International Congress of Invertebrate Pathology and Microbial Control and the 51st Annual Meeting of the Society for Invertebrate Pathology, Society for Invertebrate Pathology, Aug 2018, Gold Coast, Australia. ⟨hal-04370041⟩
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