This study involves monolayer deposits of isolated microparticles on a surface in a ventilation duct. It aims at studying the influence of the airflow pattern (an acceleration stage followed by a short steady-state period) properties on microparticle behaviour. For that purpose, the mean velocity and acceleration roles are investigated, and the evolution of the particle fraction remaining on the duct wall is analysed as a function of time. The experimental procedure involves filming microparticle deposits and monitoring airflow characteristics using constant temperature anemometry. Centre and near-wall velocities and wall shear stress are monitored using hot-wire and glue on hot-film probes, respectively. Bronze particles were used for their spherical morphology. Two particle fractions with narrow size distributions characterised by d50 of 23.3 and 31.3 μm were tested to investigate the influence of the particle size on resuspension. Particle Tracking Velocimetry (PTV), i.e., measure particle trajectories and their velocity and acceleration was carried out. Results showed that most particles resuspended during the acceleration regime for all temporal airflow patterns. It emphasises that the phenomenon must be studied through time-resolved approaches. We propose to link the physical phenomena responsible for the resuspension with the appearance of turbulence in the near-wall region.