Characterization of Soluble Cell-Free Coelomic Fluid Proteome from the Starfish Marthasterias glacialis - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement
Article Dans Une Revue Methods in Molecular Biology Année : 2022

Characterization of Soluble Cell-Free Coelomic Fluid Proteome from the Starfish Marthasterias glacialis

Résumé

Abstract Proteomics combined to advanced bioinformatics tools is acquiring a pivotal role in the comprehensive understanding of living organism’s biology, in particular for non-model organisms, which includes most marine and aquatic invertebrates. Depicting of protein composition in a whole organ/organism followed by their assembling in functional protein association networks promotes the understanding of key biological processes. Here, we provide a detailed description of the extraction procedure of cell-free coelomic fluid soluble proteins and the characterization of the proteome of the starfish Marthasterias glacialis . Due to coelomic fluid richness in glycoproteins, which complicates protein identification, extracts of soluble proteins are deglycosylated prior to tandem mass spectrometry. This experimental approach is useful at improving knowledge on the coelomic fluid physiological role and deciphering its involvement in regeneration of starfish body parts when comparing different regeneration conditions.
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Dates et versions

hal-04460177 , version 1 (15-02-2024)

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Laidson Paes Gomes, Catarina Gouveia E Silva, Jean-Charles Gaillard, Jean Armengaud, Ana Varela Coelho. Characterization of Soluble Cell-Free Coelomic Fluid Proteome from the Starfish Marthasterias glacialis. Methods in Molecular Biology, 2022, Methods in Molecular Biology, 2450, pp.583-597. ⟨10.1007/978-1-0716-2172-1_31⟩. ⟨hal-04460177⟩
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