A synthetic biology approach to transform Yarrowia lipolytica into a competitive biotechnological producer of β‐carotene - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Biotechnology and Bioengineering Year : 2017

A synthetic biology approach to transform Yarrowia lipolytica into a competitive biotechnological producer of β‐carotene

Abstract

The increasing market demands of β‐carotene as colorant, antioxidant and vitamin precursor, requires novel biotechnological production platforms. Yarrowia lipolytica , is an industrial organism unable to naturally synthesize carotenoids but with the ability to produce high amounts of the precursor Acetyl‐CoA. We first found that a lipid overproducer strain was capable of producing more β‐carotene than a wild type after expressing the heterologous pathway. Thereafter, we developed a combinatorial synthetic biology approach base on Golden Gate DNA assembly to screen the optimum promoter‐gene pairs for each transcriptional unit expressed. The best strain reached a production titer of 1.5 g/L and a maximum yield of 0.048 g/g of glucose in flask. β‐carotene production was further increased in controlled conditions using a fed‐batch fermentation. A total production of β‐carotene of 6.5 g/L and 90 mg/g DCW with a concomitant production of 42.6 g/L of lipids was achieved. Such high titers suggest that engineered Y. lipolytica is a competitive producer organism of β‐carotene.

Dates and versions

hal-04565039 , version 1 (01-05-2024)

Licence

Attribution - NoDerivatives

Identifiers

Cite

Macarena Larroude, Ewelina Celinska, Alexandre Back, Stephan Thomas, Jean‐marc Nicaud, et al.. A synthetic biology approach to transform Yarrowia lipolytica into a competitive biotechnological producer of β‐carotene. Biotechnology and Bioengineering, 2017, 115 (2), pp. 464-472. ⟨10.1002/bit.26473⟩. ⟨hal-04565039⟩
0 View
0 Download

Altmetric

Share

Gmail Facebook X LinkedIn More