A new point mutation in the HC-Pro of potato virus Y is involved in tobacco vein necrosis - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles PLoS ONE Year : 2024

A new point mutation in the HC-Pro of potato virus Y is involved in tobacco vein necrosis

Abstract

Tobacco vein necrosis (TVN) is a complex phenomenon regulated by different genetic determinants mapped in the HC-Pro protein (amino acids N 330 , K 391 and E 410 ) and in two regions of potato virus Y (PVY) genome, corresponding to the cytoplasmic inclusion (CI) protein and the nuclear inclusion protein a-protease (NIa-Pro), respectively. A new determinant of TVN was discovered in the MK isolate of PVY which, although carried the HC-Pro determinants associated to TVN, did not induce TVN. The HC-Pro open reading frame (ORF) of the necrotic infectious clone PVY N605 was replaced with that of the non-necrotic MK isolate, which differed only by one amino acid at position 392 (T 392 instead of I 392 ). The cDNA clone N605_MKHCPro inoculated in tobacco induced only weak mosaics at the systemic level, demostrating that the amino acid at position 392 is a new determinant for TVN. No significant difference in accumulation in tobacco was observed between N605 and N605_MKHCPro. Since phylogenetic analyses showed that the loss of necrosis in tobacco has occurred several times independently during PVY evolution, these repeated evolutions strongly suggest that tobacco necrosis is a costly trait in PVY.
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Dates and versions

hal-04573106 , version 1 (13-05-2024)

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Giuseppe Parrella, Benoît Moury. A new point mutation in the HC-Pro of potato virus Y is involved in tobacco vein necrosis. PLoS ONE, 2024, 19 (5), pp.e0302692. ⟨10.1371/journal.pone.0302692⟩. ⟨hal-04573106⟩
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