Benchmarking of sequencing technologies for CpG methylation detection: applications to quail and pig
Résumé
Context : Current technologies for detecting and analyzing epigenetic modifications are not yet as advanced or as cost-effective as those used for genetic analysis. This makes large-scale screening of epigenetic traits in breeding populations challenging and expensive. Advances in technology and a deeper understanding of epigenetic mechanisms will likely enhance the effectiveness and precision of epigenetic breeding in the future.
Objectives : Provide research teams with a powerful and innovative method to study methylation marks in a large number of individuals within breeding programs.
Questions : Is Whole Genome Bisulfite Sequencing (WGBS) still the gold standard for DNA methylation analysis ? Is Enzymatic Methyl-seq (EM-seq) a better reference to represent the short-read sequencing ? Technologies like Oxford Nanopore (ONT) and PacBio offer the advantage of long reads but are they powerful for detecting methylation ?
Domaines
Sciences du Vivant [q-bio]| Origine | Fichiers produits par l'(les) auteur(s) |
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