Benchmarking of sequencing technologies for CpG methylation detection: applications to quail and pig - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Conference Poster Year : 2024

Benchmarking of sequencing technologies for CpG methylation detection: applications to quail and pig

Paul Terzian
  • Function : Author
  • PersonId : 1018566
Joanna Lledo
  • Function : Author
  • PersonId : 1207875
Jules Sabban
  • Function : Author
  • PersonId : 1148557
Sophie Leroux
Julie Demars
Christine Gaspin
  • Function : Author
  • PersonId : 1219963
Denis Milan
Carole Iampietro

Abstract

Context : Current technologies for detecting and analyzing epigenetic modifications are not yet as advanced or as cost-effective as those used for genetic analysis. This makes large-scale screening of epigenetic traits in breeding populations challenging and expensive. Advances in technology and a deeper understanding of epigenetic mechanisms will likely enhance the effectiveness and precision of epigenetic breeding in the future. Objectives : Provide research teams with a powerful and innovative method to study methylation marks in a large number of individuals within breeding programs. Questions : Is Whole Genome Bisulfite Sequencing (WGBS) still the gold standard for DNA methylation analysis ? Is Enzymatic Methyl-seq (EM-seq) a better reference to represent the short-read sequencing ? Technologies like Oxford Nanopore (ONT) and PacBio offer the advantage of long reads but are they powerful for detecting methylation ?
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Dates and versions

hal-04638952 , version 1 (08-07-2024)

Identifiers

  • HAL Id : hal-04638952 , version 1

Cite

Céline Vandecasteele, Paul Terzian, Joanna Lledo, Rémy-Félix Serre, Jules Sabban, et al.. Benchmarking of sequencing technologies for CpG methylation detection: applications to quail and pig. London Calling 2024, May 2024, London, France. ⟨hal-04638952⟩
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