In-situ biomethanation shows significant potential for enhancing biogas quality. The efficiency of this process relies on various factors, including organic loading rate and hydrogen injection frequency. Experiments were carried out to evaluate the impact of different organic loading rates on hydrogen consumption rate during in-situ biomethanation. Results indicate that higher organic loading rates resulted in increased hydrogen consumption rates, with the highest value (57 mg COD/L/h) being observed at the highest OLR tested (2.75 g VS/L/d). In addition, stable hydrogen kinetics were maintained after a 12-hour hydrogen starvation period. These results provide valuable insights that can contribute to the understanding and optimisation of in-situ biomethanation. Keywords: Anaerobic digestion; In-situ biomethanation; power to gas; biogas upgrading