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Etude de quelques mécanismes de transport impliqués dans l'absorptin et la remobilisation de l'azote chez le peuplier

Abstract : This work concerns the identification of molecular mechanisms involved in the absorption and transport of different nitrogen sources, ammonium and amino acids, in poplar. The first part of the work was based on bioinformatic analyses to identify gene modeles coding for nitrogen compound transporters. The second part of my work dealt with the characterization of poplar AMT family which comprised 14 members, some of which presented specific expression pattern, such as AMT1;2 in roots and AMT3;1 in senescing leaves. The third and fourth parts of my work focused on transport mechanisms involved in nitrogen remobilization during autumn and spring. We studied more particularly two families of amino acid transporters, the AAP family and the CAT family. A strong accumulation of arginine was observed in stem during senescence. Glutamine could be preferentially used as a transport component from senescing to perennial tissues and could be preferentially used as nitrogen source for arginine synthesis in stem. Furthermore, CAT11 expression was subjected to high variations in stem, which could be related to total amino acid content and arginine particularly. Moreover, we showed that the specific expression of a putative amino acid transporter AAP11 in bud before bud-burst is linked to the formation of protoxylem cells in vegetative buds.
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  • HAL Id : tel-02814092, version 1
  • PRODINRA : 277480

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Jérémy Couturier. Etude de quelques mécanismes de transport impliqués dans l'absorptin et la remobilisation de l'azote chez le peuplier. Sciences du Vivant [q-bio]. Université Henri Poincaré (Nancy 1), 2007. Français. ⟨tel-02814092⟩

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