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Analyse des protéines du lait des Camélidés par approches protéomique et moléculaire

Abstract : Milk is known to provide an excellent nutrition and protection against infection. It contains a range of soluble and cellular components which provide essential nutriments. Milks from different species differ significantly as to their protective proteins content which plays a role in the protection to the developing animal and/or human. In spite of their richness and their considerable production, dromedary and llama milks remain products relatively not much consumed and transformed because insufficiently studied and emphasized. The objective of this study was to investigate and compare the protein composition of the different fractions of llama and dromedary milk. Skimmed llama and dromedary milk proteins were first characterized by a two dimensional separation technique coupling Reverse Phase High Pressure Liquid Chromatography in the first dimension with sodium dodecyl sulphate polyacrylamide gel electrophoresis in the second dimension (RP-HPLC-SDS-PAGE). Milk proteins: caseins (αs1, αs2,β and κ caseins.) and some whey proteins like α-lactalbumin, lactoferin and serum albumin were identified using peptide mass fingerprinting. Llama and dromedary proteins milk were also characterized by on-line Reverse Phase High Pressure Liquid Chromatography/Electrospray Ionization Mass Spectrometry (LC-ESI-MS) analysis. This approach allowed attributing precise molecular masses for most of the previously MS-identified milk proteins. We reported also the successful use of the Two-Dimensional gel Electrophoresis (2-DE) tool to identify over 45 protein spots present in the dromedary whey milk. De novo sequencing of the llama α-lactalbumin protein by Liquid Chromatography coupled with tandem Mass Spectrometry (LC-MS/MS) showed the occurrence of two amino acid substitutions (R62L/I and K89L/I). Because in camelids, nothing is known about the milk fat globule membrane (MFGM), we used 1D-LC-MS/MS to identify 322 and 187 functional groups of proteins associated with dromedary and llama milk respectively.
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Contributor : Christelle Cebo <>
Submitted on : Thursday, October 1, 2020 - 1:42:35 PM
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Christelle Cebo, Besma Saadaoui. Analyse des protéines du lait des Camélidés par approches protéomique et moléculaire. Sciences du Vivant [q-bio]. Université de Tunis - ECOLE SUPERIEURE DES SCIENCES ET TECHNIQUES DE TUNIS, 2015. Français. ⟨tel-02954861⟩

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