Skip to Main content Skip to Navigation
Theses

Contribution à l'étude de l'expression du génome racinaire de Vicia faba: analyse de l'expression différentielle des mRNA

Abstract : In an attempt to determine the extent of gene expression during cell differentiation in the root system, we have used the natural transition from meristematic to mature stage which is accompagnied by the cessation of cell division, cell elongation, and histodifferentiation. Using cDNA probes, the polysomal polyadenylated RNA populations of Vicia faba meristematic, elongating and mature root cells, were analysed and compared, with respect to complexity, abundance distribution, and sequence representation. These cells express 12500, 15800 and 15200 sequences, respectively, of an average size of 1300 nucleotides, distributed in three frequency classes. Transition from the meritematic to the elongating stage is coordinated with the disappearance of 25-30 of the abundant RNA species (2000 copies per cell) and with the appearance of new transcripts corresponding to the rare class (about 4 copies per cell). These new transcripts represent 12.5% of the mass of the mRNA during the elongating stage and are quantitatively modulated during the transition to the mature stage. Thus 80 to 85% of the polysomal polyadenylated RNA species expressed in elongating or mature cells are common to the three cell types.
Document type :
Theses
Complete list of metadata

https://hal.inrae.fr/tel-03156897
Contributor : Victor Vaillant <>
Submitted on : Tuesday, March 2, 2021 - 5:31:07 PM
Last modification on : Tuesday, March 23, 2021 - 10:56:02 PM

Identifiers

  • HAL Id : tel-03156897, version 1

Collections

Citation

Victor Vaillant. Contribution à l'étude de l'expression du génome racinaire de Vicia faba: analyse de l'expression différentielle des mRNA. Biologie végétale. Université Paris 7, 1981. Français. ⟨tel-03156897⟩

Share

Metrics

Record views

12