Regulation of LPCAT3 by LXR. - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Gene Year : 2011

Regulation of LPCAT3 by LXR.

O. Demeure
  • Function : Author
Frédéric Lecerf
C. Duby
  • Function : Author
S. Ducheix
  • Function : Author
Hervé Guillou
Sandrine Lagarrigue


In this work we analyzed the transcriptome profiles of chicken hepatoma cells (LMH) in response to T0901317, a pharmacological agonist of the liver X receptor (LXR). Through an in silico search for LXRE (LXR response element) consensus sequences in the promoter of genes whose expression was shown to be sensitive to TO901317, we identified a LXRE in the promoter of the LPCAT3 (lysophosphatidylcholine acyltransferase 3). This motif is highly conserved between species. We further investigated the regulation of this gene and showed that the expression of LPCAT3 was induced both in chicken and human hepatoma cells (LMH and HuH-7, respectively) in response to T0901317. Transactivation and electrophoretic mobility shift assays allowed us to locate a functional LXRE in the chicken LPCAT3 promoter. Altogether these data evidence for the first time that the chicken LPCAT3 gene is a direct target of LXR and therefore suggest a new role for LXR in phospholipid homeostasis.

Dates and versions

hal-00729271 , version 1 (07-09-2012)



O. Demeure, Frédéric Lecerf, C. Duby, Colette Désert, S. Ducheix, et al.. Regulation of LPCAT3 by LXR.. Gene, 2011, 470 (1-2), pp.7-11. ⟨10.1016/j.gene.2010.09.002⟩. ⟨hal-00729271⟩
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