Proficiency of PCR in hospital settings for nonculture diagnosis of invasive meningococcal infections. - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Clinical Laboratory Year : 2012

Proficiency of PCR in hospital settings for nonculture diagnosis of invasive meningococcal infections.

Ludovic Lemée
  • Function : Author
Jean-Louis Pons
  • Function : Author

Abstract

BACKGROUND: Meningococcal meningitis requires rapid diagnosis and immediate management which is enhanced by the use of PCR for the ascertainment of these infections. However, its use is still restricted to reference laboratories. METHODS: We conducted an inter-laboratory study to assess the implementation and the performance of PCR in ten French hospital settings in 2010. RESULTS: Our data are in favour of this implementation. Although good performance was obtained in identifying Neisseria meningitidis positive samples, the main issue was reported in identifying other species (Streptococcus pneumoniae and Haemophilus influenzae) which are also involved in bacterial meningitis cases. CONCLUSIONS: Several recommendations are required and, mainly, PCR should target the major etiological agents (N. meningitidis, S. pneumonia, and H. influenzae) of acute bacterial meningitis. Moreover, PCR should predict the most frequent serogroups of Neisseria meningitidis according to local epidemiology.
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Dates and versions

hal-00738980 , version 1 (05-10-2012)

Identifiers

  • HAL Id : hal-00738980 , version 1
  • PRODINRA : 352491
  • PUBMED : 22582511
  • WOS : 000302504900021

Cite

Eva Hong, Olivier Barraud, Philippe Bidet, Edouard Bingen, Nicolas Blondiaux, et al.. Proficiency of PCR in hospital settings for nonculture diagnosis of invasive meningococcal infections.. Clinical Laboratory, 2012, 58 (3-4), pp.343-6. ⟨hal-00738980⟩
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