Skip to Main content Skip to Navigation
New interface
Journal articles

Who is the puppet master? Replication of a parasitic wasp-associated virus correlates with host behaviour manipulation

Abstract : Many parasites modify their host behaviour to improve their own transmission and survival, but the proximate mechanisms remain poorly understood. An original model consists of the parasitoid Dinocampus coccinellae and its coccinellid host, Coleomegilla maculata; during the behaviour manipulation, the parasitoid is not in contact with its host anymore.We report herein the discovery and characterization of a new RNA virus of the parasitoid (D. coccinellae paralysis virus, DcPV). Using a combination of RT-qPCR and transmission electron microscopy, we demonstrate that DcPV is stored in the oviduct of parasitoid females, replicates in parasitoid larvae and is transmitted to the host during larval development. Next, DcPV replication in the host’s nervous tissue induces a severe neuropathy and antiviral immune response that correlate with the paralytic symptoms characterizing the behaviour manipulation. Remarkably, virus clearance correlates with recovery of normal coccinellid behaviour. These results provide evidence that changes in ladybeetle behaviour most likely result from DcPV replication in the cerebral ganglia rather than by manipulation by the parasitoid. This offers stimulating prospects for research on parasitic manipulation by suggesting for the first time that behaviour manipulation could be symbiont-mediated.
Document type :
Journal articles
Complete list of metadata
Contributor : Anne Modat Connect in order to contact the contributor
Submitted on : Tuesday, January 12, 2021 - 11:08:52 AM
Last modification on : Tuesday, December 6, 2022 - 3:12:51 AM
Long-term archiving on: : Tuesday, April 13, 2021 - 6:21:12 PM


Publisher files allowed on an open archive



Nolwenn M. Dheilly, Fanny Maure, Marc Ravallec, Richard Galinier, Josée Doyon, et al.. Who is the puppet master? Replication of a parasitic wasp-associated virus correlates with host behaviour manipulation. Proceedings of the Royal Society B: Biological Sciences, 2015, 282 (1803), pp.20142773. ⟨10.1098/rspb.2014.2773⟩. ⟨hal-01141139⟩



Record views


Files downloads