Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Isolation, Characterization, Kinetics, and Enzymatic and Nonenzymatic Microbicidal Activities of a Novel c-Type Lysozyme from Plasma of Schistocerca gregaria (Orthoptera: Acrididae)

Abstract : A protein, designated as Sgl, showing a muramidase lytic activity to the cell wall of the Gram-positive bacterium Micrococcus lysodeikticus was isolated for the first time from plasma of Escherichia coli-immunized fifth instar Schistocerca gregaria. The isolated Sgl was detected as a single protein band, on both native-and SDS-PAGE, has a molecular weight of similar to 15.7 kDa and an isoelectric point (pI) of ca 9.3 and its antiserum has specifically recognized its isolated form. Fifty-nine percentage of Sgl lytic activity was recovered in the isolated fractions and yielded ca 126-fold increase in specific activity than that of the crude. The partial N-terminal amino acid sequence of the Sgl has 55 and 40% maximum identity with Bombyx mori and Gallus gallus c-type lysozymes, respectively. The antibacterial activity against the Gram-positive and the Gram-negative bacteria were comparatively stronger than that of the hen egg white lysozyme (HEWL). The detected Sgl poration to the inner membrane that reach a maximum ability after 3 h was suggested to operate as a nonenzymatic mechanism for Gram-negative bacterial cell lysis, as tested in a permease-deficient E. coli, ML 35 strain. Sgl showed a maximal muramidase activity at pH 6.2, 30-50 degrees C, and 0.05M Ca2+ or Mg2+; and has a K-m of 0.5 mu g/ml and a V-max of 0.518 with M. lysodeikticus as a substrate. The Sgl displayed a chitinase activity against chitin with a K-m of 0.93 mg/ml and a V-max of 1.63.
Type de document :
Article dans une revue
Liste complète des métadonnées

Littérature citée [67 références]  Voir  Masquer  Télécharger

https://hal.archives-ouvertes.fr/hal-01165742
Déposant : Archive Ouverte Prodinra <>
Soumis le : mardi 21 juillet 2015 - 20:10:26
Dernière modification le : mardi 2 février 2021 - 18:08:02
Archivage à long terme le : : mercredi 26 avril 2017 - 07:52:03

Fichier

Elmogy 2015 J. of Insect Scien...
Accord explicite pour ce dépôt

Identifiants

Collections

Citation

Mohamed Elmogy, Taha T. M. Bassal, Hesham A. Yousef, Moataza A. Dorrah, Amr A. Mohamed, et al.. Isolation, Characterization, Kinetics, and Enzymatic and Nonenzymatic Microbicidal Activities of a Novel c-Type Lysozyme from Plasma of Schistocerca gregaria (Orthoptera: Acrididae). Journal of Insect Science, Oxford University Press, 2015, 15 (1), 10 p. ⟨10.1093/jisesa/iev038⟩. ⟨hal-01165742v2⟩

Partager

Métriques

Consultations de la notice

1179

Téléchargements de fichiers

314