Estimation from Moments Measurements for Amyloid Depolymerisation - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue Journal of Theoretical Biology Année : 2016

Estimation from Moments Measurements for Amyloid Depolymerisation

Résumé

Estimating reaction rates and size distributions of protein polymers is an important step for understanding the mechanisms of protein misfolding and aggregation, a key feature for amyloid diseases. This study aims at {setting} this framework problem when the experimental measurements consist in the time-dynamics of a moment of the population (\emph{i.e.} for instance the total polymerised mass, as in Thioflavine T measurements, or the second moment measured by Static Light Scattering). We propose a general methodology, and we solve the problem theoretically and numerically in the case of a depolymerising system. We then apply our method to experimental data of degrading oligomers, and conclude that smaller aggregates of ovPrP protein should be more stable than larger ones. This has an important biological implication, since it is commonly admitted that small oligomers constitute the most cytotoxic species during prion misfolding process.
Fichier principal
Vignette du fichier
ADMR_paper.pdf (3.47 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-01248255 , version 1 (24-12-2015)

Identifiants

Citer

Aurora Armiento, Marie Doumic, Philippe Moireau, Human Rezaei. Estimation from Moments Measurements for Amyloid Depolymerisation. Journal of Theoretical Biology, 2016, 397, pp.68 - 88. ⟨10.1016/j.jtbi.2016.02.037⟩. ⟨hal-01248255⟩
650 Consultations
222 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More