Enzymatic production of bioactive docosahexaenoic acid phenolic ester - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Food Chemistry Year : 2015

Enzymatic production of bioactive docosahexaenoic acid phenolic ester


Docosahexaenoic acid (DHA) is increasingly considered for its health benefits. However, its use as functional food ingredient is still limited by its instability. In this work, we developed an efficient and solvent-free bioprocess for the synthesis of a phenolic ester of DHA. A fed-batch process catalyzed by Candida antarctica lipase B was optimised, leading to the production of 440 g/L vanillyl ester (DHA-VE). Structural characterisation of the purified product indicated acylation of the primary OH group of vanillyl alcohol. DHA-VE exhibited a high radical scavenging activity in acellular systems. In vivo experiments showed increased DHA levels in erythrocytes and brain tissues of mice fed DHA-VE-supplemented diet. Moreover, in vitro neuroprotective properties of DHA-VE were demonstrated in rat primary neurons exposed to amyloid-beta oligomers. In conclusion, DHA-VE synergized the main beneficial effects of two common natural biomolecules and therefore appears a promising functional ingredient for food applications.

Dates and versions

hal-01267210 , version 1 (04-02-2016)



Mohamed H. Roby, Ahmad Allouche, Layal Dahdou, Vanessa C. de Castro, Paulo H. Alves Da Silva, et al.. Enzymatic production of bioactive docosahexaenoic acid phenolic ester. Food Chemistry, 2015, 171, pp.397-404. ⟨10.1016/j.foodchem.2014.09.028⟩. ⟨hal-01267210⟩
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