The Redox Status of Cancer Cells Supports Mechanisms behind the Warburg Effect - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue Metabolites Année : 2016

The Redox Status of Cancer Cells Supports Mechanisms behind the Warburg Effect

Résumé

To better understand the energetic status of proliferating cells, we have measured the intracellular pH (pHi) and concentrations of key metabolites, such as adenosine triphosphate (ATP), nicotinamide adenine dinucleotide (NAD), and nicotinamide adenine dinucleotide phosphate (NADP) in normal and cancer cells, extracted from fresh human colon tissues. Cells were sorted by elutriation and segregated in different phases of the cell cycle (G0/G1/S/G2/M) in order to study their redox (NAD, NADP) and bioenergetic (ATP, pHi) status. Our results show that the average ATP concentration over the cell cycle is higher and the pHi is globally more acidic in normal proliferating cells. The NAD + /NADH and NADP + /NADPH redox ratios are, respectively, five times and ten times higher in cancer cells compared to the normal cell population. These energetic differences in normal and cancer cells may explain the well-described mechanisms behind the Warburg effect. Oscillations in ATP concentration, pHi, NAD + /NADH, and NADP + /NADPH ratios over one cell cycle are reported and the hypothesis addressed. We also investigated the mitochondrial membrane potential (MMP) of human and mice normal and cancer cell lines. A drastic decrease of the MMP is reported in cancer cell lines compared to their normal counterparts. Altogether, these results strongly support the high throughput aerobic glycolysis, or Warburg effect, observed in cancer cells.
Fichier principal
Vignette du fichier
metabolites-06-00033-v2.pdf (1.56 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-01417967 , version 1 (16-12-2016)

Licence

Paternité - Partage selon les Conditions Initiales

Identifiants

Citer

Jorgelindo da Veiga Moreira, Minoo Hamraz, Mohammad Abolhassani, Erwan Bigan, Sabine Pérès, et al.. The Redox Status of Cancer Cells Supports Mechanisms behind the Warburg Effect. Metabolites, 2016, 6 (4), 12 p. ⟨10.3390/metabo6040033⟩. ⟨hal-01417967⟩
610 Consultations
187 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More