Carotenoid gene expression explains the difference of carotenoid accumulation in carrot root tissues - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Planta Year : 2016

Carotenoid gene expression explains the difference of carotenoid accumulation in carrot root tissues

Laura Hartmann
  • Function : Author
Cécile Dubois-Laurent
  • Function : Author
Ralf Welsch
  • Function : Author
Sébastien Huet
  • Function : Author
Latifa Hamama
  • Function : Author
Mathilde Briard
Didier Peltier
  • Function : Author
  • PersonId : 1203327
Séverine Gagné
  • Function : Author
Emmanuel E. Geoffriau

Abstract

The carrot root is well divided into two different tissues separated by vascular cambium: the secondary phloem and xylem. The equilibrium between these two tissues represents an important issue for carrot quality, but the knowledge about the respective carotenoid accumulation is sparse. The aim of this work was (i) to investigate if variation in carotenoid biosynthesis gene expression could explain differences in carotenoid content in phloem and xylem tissues and (ii) to investigate if this regulation is differentially modulated in the respective tissues by water-restricted growing conditions. In this work, five carrot genotypes contrasting by their root color were studied in control and water-restricted conditions. Carotenoid content and the relative expression of 13 genes along the carotenoid biosynthesis pathway were measured in the respective tissues. Results showed that in orange genotypes and the purple one, carotenoid content was higher in phloem compared to xylem. For the red one, no differences were observed. Moreover, in control condition, variations in gene expression explained the different carotenoid accumulations in both tissues, while in water-restricted condition, no clear association between gene expression pattern and variations in carotenoid content could be detected except in orange-rooted genotypes. This work shows that the structural aspect of carrot root is more important for carotenoid accumulation in relation with gene expression levels than the consequences of expression changes upon water restriction.

No file

Dates and versions

hal-01426413 , version 1 (04-01-2017)

Licence

Attribution - ShareAlike

Identifiers

Cite

Florent Perrin, Laura Hartmann, Cécile Dubois-Laurent, Ralf Welsch, Sébastien Huet, et al.. Carotenoid gene expression explains the difference of carotenoid accumulation in carrot root tissues. Planta, 2016, 245 (4), pp.1-11. ⟨10.1007/s00425-016-2637-9⟩. ⟨hal-01426413⟩
188 View
0 Download

Altmetric

Share

Gmail Facebook X LinkedIn More