Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Identification and application of keto acids transporters in Yarrowia lipolytica

Abstract : Production of organic acids by microorganisms is of great importance for obtaining building-block chemicals from sustainable biomass. Extracellular accumulation of organic acids involved a series of transporters, which play important roles in the accumulation of specific organic acid while lack of systematic demonstration in eukaryotic microorganisms. To circumvent accumulation of by-product, efforts have being orchestrated to carboxylate transport mechanism for potential clue in Yarrowia lipolytica WSH-Z06. Six endogenous putative transporter genes, YALI0B19470g, YALI0C15488g, YALI0C21406g, YALI0D24607g, YALI0D20108g and YALI0E32901g, were identified. Transport characteristics and substrate specificities were further investigated using a carboxylate-transport-deficient Saccharomyces cerevisiae strain. These transporters were expressed in Y. lipolytica WSH-Z06 to assess their roles in regulating extracellular keto acids accumulation. In a Y. lipolytica T1 line over expressing YALI0B19470g, α-ketoglutarate accumulated to 46.7 g\textperiodcenteredL(-1), whereas the concentration of pyruvate decreased to 12.3 g\textperiodcenteredL(-1). Systematic identification of these keto acids transporters would provide clues to further improve the accumulation of specific organic acids with higher efficiency in eukaryotic microorganisms.
Type de document :
Article dans une revue
Liste complète des métadonnées

Littérature citée [48 références]  Voir  Masquer  Télécharger

https://hal-agroparistech.archives-ouvertes.fr/hal-01564919
Déposant : Armelle Sielinou <>
Soumis le : mercredi 27 mai 2020 - 07:55:50
Dernière modification le : jeudi 11 juin 2020 - 04:22:23

Fichier

2015_Guo_Scientific Reports_1....
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Citation

Hongwei Guo, Peiran Liu, Catherine Madzak, Guocheng Du, Jingwen Zhou, et al.. Identification and application of keto acids transporters in Yarrowia lipolytica. Scientific Reports, Nature Publishing Group, 2015, 5, pp.8138. ⟨10.1038/srep08138⟩. ⟨hal-01564919⟩

Partager

Métriques

Consultations de la notice

124

Téléchargements de fichiers

27