Priming and polymerization of a bacterial contractile tail structure. 1" 2" 17" - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Nature Year : 2016

Priming and polymerization of a bacterial contractile tail structure. 1" 2" 17"

Pierre Legrand
Rémi Fronzes
Tam Mignot

Abstract

Contractile tails are composed of an inner tube wrapped by an outer sheath assembled in an extended, metastable conformation that stores mechanical energy necessary for its contraction. Contraction is used to propel the rigid inner tube towards target cells for DNA or toxin delivery. Although recent studies have revealed the structure of the Type VI secretion system contractile sheath, the mechanisms by which its polymerization is controlled and coordinated with inner tube assembly remain unsolved. In this study, we report that the starfish-like TssA dodecameric complex interacts with tube and sheath components. Fluorescence microscopy experiments revealed that TssA binds first to the T6SS membrane core complex and then initiates tail polymerization. TssA remains at the tip of the growing structure and incorporates new tube and sheath blocks. Based on these results, we propose that TssA primes and coordinates tail tube and sheath biogenesis.
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Dates and versions

hal-01778575 , version 1 (25-04-2018)

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Abdelrahim Zoued, Eric Durand, Yannick R Brunet, Silvia Spinelli, Badreddine Douzi, et al.. Priming and polymerization of a bacterial contractile tail structure. 1" 2" 17". Nature, 2016, 531 (7592), pp.59 - 63. ⟨10.1038/nature17182⟩. ⟨hal-01778575⟩
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