De novo assembly of the complex genome of Nippostrongylus brasiliensis using MinION long reads - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles BMC Biology Year : 2018

De novo assembly of the complex genome of Nippostrongylus brasiliensis using MinION long reads

Abstract

Eukaryotic genome assembly remains a challenge in part due to the prevalence of complex DNA repeats. This is a particularly acute problem for holocentric nematodes because of the large number of satellite DNA sequences found throughout their genomes. These have been recalcitrant to most genome sequencing methods. At the same time, many nematodes are parasites and some represent a serious threat to human health. There is a pressing need for better molecular characterization of animal and plant parasitic nematodes. The advent of long-read DNA sequencing methods offers the promise of resolving complex genomes.
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hal-02094619 , version 1 (24-04-2019)

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David W Eccles, Jodie Chandler, Mali Camberis, Bernard Henrissat, Sergey Koren, et al.. De novo assembly of the complex genome of Nippostrongylus brasiliensis using MinION long reads. BMC Biology, 2018, 16 (1), pp.6. ⟨10.1186/s12915-017-0473-4⟩. ⟨hal-02094619⟩
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