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Journal Articles Interface Focus Year : 2019

Harnessing glycoenzyme engineering for synthesis of bioactive oligosaccharides

Abstract

Combined with chemical synthesis, the use of glycoenzyme biocatalysts has shown great synthetic potential over recent decades owing to their remarkable versatility in terms of substrates and regio- and stereoselectivity that allow structurally controlled synthesis of carbohydrates and glycoconjugates. Nonetheless, the lack of appropriate enzymatic tools with requisite properties in the natural diversity has hampered extensive exploration of enzyme-based synthetic routes to access relevant bioactive oligosaccharides, such as cell-surface glycans or prebiotics. With the remarkable progress in enzyme engineering, it has become possible to improve catalytic efficiency and physico-chemical properties of enzymes but also considerably extend the repertoire of accessible catalytic reactions and tailor novel substrate specificities. In this review, we intend to give a brief overview of the advantageous use of engineered glycoenzymes, sometimes in combination with chemical steps, for the synthesis of natural bioactive oligosaccharides or their precursors. The focus will be on examples resulting from the three main classes of glycoenzymes specialized in carbohydrate synthesis: glycosyltransferases, glycoside hydrolases and glycoside phosphorylases.
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Dates and versions

hal-02146106 , version 1 (23-11-2020)

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Mounir Benkoulouche, Régis Fauré, Magali Remaud Simeon, Claire Moulis, Isabelle André. Harnessing glycoenzyme engineering for synthesis of bioactive oligosaccharides. Interface Focus, 2019, 9 (2), pp.20180069. ⟨10.1098/rsfs.2018.0069⟩. ⟨hal-02146106⟩
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