Persistent production of an integrase-deleted HIV-1 variant with no resistance mutation and wild-type proviral DNA in a treated patient - Archive ouverte HAL Access content directly
Journal Articles AIDS RESEARCH AND HUMAN RETROVIRUSES Year : 2015

Persistent production of an integrase-deleted HIV-1 variant with no resistance mutation and wild-type proviral DNA in a treated patient

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1
M. A. Trabaud
  • Function : Author
L. Cotte
  • Function : Author
J. Saison
  • Function : Author
C. Ramiere
  • Function : Author
Corinne Ronfort
F. Venet
  • Function : Author
J. C. Tardy
  • Function : Author
G. Monneret
  • Function : Author
P. Andre
  • Function : Author

Abstract

An HIV-infected patient presenting an unexpected viral escape under combined antiretroviral treatment is described. The virus isolated from plasma contained a large deletion in the HIV-1 integrase gene but no known resistance mutation. Nested polymerase chain reactions (PCRs) with patient virus integrase-specific primers and probes were developed and used to detect the mutant from plasma, blood, rectal biopsies, and sperm. The variant progressively emerged during a period of therapy-induced virosuppression, and persisted at a low but detectable level for at least 5 years. Surprisingly, proviral DNA from lymphocytes, rectal cells, and sperm cells was, and remained, mainly wild type. Cellular HIV RNA with the deletion was detected only once from the rectum. The origin and mechanisms underlying this so far not described production at a detectable level are largely hypothetical. This observation raised concern about the ability of defective viruses to spread.

Dates and versions

hal-02192928 , version 1 (24-07-2019)

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M. A. Trabaud, L. Cotte, J. Saison, C. Ramiere, Corinne Ronfort, et al.. Persistent production of an integrase-deleted HIV-1 variant with no resistance mutation and wild-type proviral DNA in a treated patient. AIDS RESEARCH AND HUMAN RETROVIRUSES, 2015, 31 (1), pp.142-9. ⟨10.1089/AID.2014.0129⟩. ⟨hal-02192928⟩
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