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A standardized toolkit for genetic engineering of CTG clade yeasts

Tatiana Defosse 1, 2 Vincent Courdavault 2 Alix Coste 3 Marc Clastre 2 Thomas Duge de Bernonville 2 Charlotte Godon 1 Patrick Vandeputte 1, 4 Arnaud Lanoue 2 Antoine Touzé 5 Tomas Linder 6 Samir Droby 7 Carlos Rosa 8 Dominique Sanglard 3 Christophe d'Enfert 9 Jean-Philippe Bouchara 1, 4 Nathalie Giglioli-Guivarc'H 2 Nicolas Papon 1, * 
* Corresponding author
1 GEIHP - Groupe d'Étude des Interactions Hôte-Pathogène
2 BBV EA 2106 - Biomolécules et biotechnologies végétales
3 UNIL - Université de Lausanne
4 CHU Angers - Centre Hospitalier Universitaire d'Angers
5 UMR ISP - Infectiologie et Santé Publique
6 SLU - Swedish University of Agricultural Sciences
7 Agricultural Research Organization - the Volcani Center
8 UFMG - Universidade Federal de Minas Gerais [Belo Horizonte]
9 BPF - Biologie et Pathogénicité fongiques
Abstract : We have developed a series of synthetic constructs suitable to genetically manipulate a broad range of yeast species belonging to the fungal CTG clade. This molecular toolbox notably allows heterologous gene expression, single or dual fluorescence labeling and construction of luciferase-expressing strains for bioluminescence imaging.
Keywords : Standardization Synthetic construct β-galactosidase. Candida Fluorescent proteins Luciferase
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Journal articles
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https://hal.univ-angers.fr/hal-02406599
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Submitted on : Thursday, December 12, 2019 - 10:57:20 AM
Last modification on : Thursday, April 21, 2022 - 3:30:36 AM

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PASTEUR | UNIV-TOURS | UNIV-ANGERS | INRA | UNAM | INRAE | BBV | INFECTIOLOGIE-SANTE-PUBLIQUE

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Tatiana Defosse, Vincent Courdavault, Alix Coste, Marc Clastre, Thomas Duge de Bernonville, et al.. A standardized toolkit for genetic engineering of CTG clade yeasts. Journal of Microbiological Methods, Elsevier, 2018, 144, pp.152-156. ⟨10.1016/j.mimet.2017.11.015⟩. ⟨hal-02406599⟩

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