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A standardized toolkit for genetic engineering of CTG clade yeasts

Abstract : We have developed a series of synthetic constructs suitable to genetically manipulate a broad range of yeast species belonging to the fungal CTG clade. This molecular toolbox notably allows heterologous gene expression, single or dual fluorescence labeling and construction of luciferase-expressing strains for bioluminescence imaging.
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https://hal.univ-angers.fr/hal-02406599
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Submitted on : Thursday, December 12, 2019 - 10:57:20 AM
Last modification on : Thursday, April 21, 2022 - 3:30:36 AM

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Tatiana Defosse, Vincent Courdavault, Alix Coste, Marc Clastre, Thomas Duge de Bernonville, et al.. A standardized toolkit for genetic engineering of CTG clade yeasts. Journal of Microbiological Methods, Elsevier, 2018, 144, pp.152-156. ⟨10.1016/j.mimet.2017.11.015⟩. ⟨hal-02406599⟩

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