CKIP-1 regulates mammalian and zebrafish myoblast fusion - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Journal of Cell Science Year : 2012

CKIP-1 regulates mammalian and zebrafish myoblast fusion

Alexandre Guiraud
Fanny Pilot-Storck
Marc Vidal


Multinucleated muscle fibres arise by fusion of precursor cells called myoblasts. We previously showed that CKIP-1 ectopic expression in C2C12 myoblasts increased cell fusion. In this work, we report that CKIP-1 depletion drastically impairs C2C12 myoblast fusion in vitro and in vivo during zebrafish muscle development. Within developing fast-twich myotome, Ckip-1 localises at the periphery of fast precursor cells, closed to the plasma membrane. Unlike wild-type myoblasts that form spatially arrayed multinucleated fast myofibres, Ckip-1-deficient myoblasts show a drastic reduction in fusion capacity. A search for CKIP-1 binding partners identified the ARPC1 subunit of Arp2/3 actin nucleation complex essential for myoblast fusion. We demonstrate that CKIP-1, through binding to plasma membrane phosphoinositides via its PH domain, regulates cell morphology and lamellipodia formation by recruiting the Arp2/3 complex at the plasma membrane. These results establish CKIP-1 as a regulator of cortical actin that recruits the Arp2/3 complex at the plasma membrane essential for muscle precursor elongation and fusion.
Fichier principal
Vignette du fichier
CKIP1bis_1.pdf (3.31 Mo) Télécharger le fichier
Origin Files produced by the author(s)

Dates and versions

hal-02646989 , version 1 (29-05-2020)



Dominique Baas, Sabine Caussanel-Boude, Alexandre Guiraud, Frederico Calhabeu, Emilie Delaune, et al.. CKIP-1 regulates mammalian and zebrafish myoblast fusion. Journal of Cell Science, 2012, 125 (Pt16), pp.3790-3800. ⟨10.1242/jcs.101048⟩. ⟨hal-02646989⟩
24 View
51 Download



Gmail Mastodon Facebook X LinkedIn More