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Specific inhibition of biohydrogen-producing Clostridium sp after dilute-acid pretreatment of sunflower stalks

Abstract : Dilute-acid pretreatments are commonly used to solubilize holocelluloses of lignocellulosic materials and represent a promising route to enhance biohydrogen production by dark fermentation. Besides the soluble sugars released, furan derivatives, such as furfural and 5-HMF, as well as phenolic compounds can accumulate in dilute-acid hydrolyzates and that may affect fermentative microbial populations. In this study, biohydrogen production from glucose (5 g VS L-1) in batch tests was investigated in presence of increasing volumes (0% - control, 3.75%, 7.5%, 15% and 35% (v/v)) of dilute acid hydrolyzate generated from sunflower stalks (170 degrees C, 1 h, 4 g HCl/100 gTS). A sharp decrease of the hydrogen yield was observed from 2.04 mol H-2 mol(-1) (eq. hexose initial) in the control to 0 mol H-2 mol(-1) (eq. hexose) (initial) for volumes higher than 15% of added hydrolyzate. Although acetate and butyrate were the main end-products found in the control, ethanol and lactate accumulated accordingly with the increasing addition of hydrolyzate. A clear shift of dominant microbial populations from Clostridium sp. to Sporolactobacillus sp. was concomitantly observed, suggesting a specific inhibition of the biohydrogen-producing bacteria by adding increasing volumes of hydrolyzates.
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https://hal.inrae.fr/hal-02650967
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Submitted on : Friday, May 29, 2020 - 3:22:45 PM
Last modification on : Friday, February 5, 2021 - 4:02:57 AM

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Florian Monlau, Quentin Aemig, Eric Trably, Jérôme Hamelin, Jean-Philippe Steyer, et al.. Specific inhibition of biohydrogen-producing Clostridium sp after dilute-acid pretreatment of sunflower stalks. International Journal of Hydrogen Energy, Elsevier, 2013, 38 (28), pp.12273 - 12282. ⟨10.1016/j.ijhydene.2013.07.018⟩. ⟨hal-02650967⟩

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