A method for the medium-term Storage of plant tissue samples at room temperature and successive cycles of DNA extraction - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Plant Molecular Biology Reporter Year : 2006

A method for the medium-term Storage of plant tissue samples at room temperature and successive cycles of DNA extraction

Abstract

Based on the protocol originally described by Stein et al. (2001), we have developed a method that allows for medium-term conservation at room temperature of wheat tissue samples to use for DNA extraction. DNA quality was suitable for analysis by PCR and Southern hybridization, even after 2 months of storage at room temperature. This method allows successive DNA re-extractions from a previously extracted sample and maximization of the DNA yield that can be recovered from precious samples. This method has applications for conservation of leaf samples and management of DNA extraction. Our method can help improve data recovery in many plant molecular genetics research projects.

Domains

Biotechnology

Dates and versions

hal-02664252 , version 1 (31-05-2020)

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Caroline C. Tassy, Catherine C. Feuillet, Pierre P. Barret. A method for the medium-term Storage of plant tissue samples at room temperature and successive cycles of DNA extraction. Plant Molecular Biology Reporter, 2006, 24, pp.247a-247f. ⟨10.1007/BF02914064⟩. ⟨hal-02664252⟩
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