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Genetic engineering of the beta-oxidation pathway in the yeast Yarrowia lipolytica to increase the production of aroma compounds

Abstract : The yeast Yarrowia lipolytica possesses five acyl-CoA oxidases (Aox1 p to 5), the enzyme catalysing the first reaction of P-oxidation. The understanding of the specific role of each acyl-CoA oxidase is important to construct a yeast strain growing at a good rate and able to produce without degrading the aroma compound gamma-decalactone. In this study we observed that Aox4p exhibits a slight activity on a broad spectrum of substrates and that it is involved in lactone degradation. We constructed a strain lacking this activity. Its growth was only slightly altered and it produced 10 times more lactone than the wild type in 48 h.
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https://hal.inrae.fr/hal-02680321
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Submitted on : Sunday, May 31, 2020 - 10:13:39 PM
Last modification on : Friday, June 12, 2020 - 10:43:26 AM

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Anne Groguenin, Yves Waché, Erandi Escamilla Garcia, Mario Aguedo, Florence Husson, et al.. Genetic engineering of the beta-oxidation pathway in the yeast Yarrowia lipolytica to increase the production of aroma compounds. Journal of Molecular Catalysis B: Enzymatic, Elsevier, 2004, 28 (2-3), pp.75-79. ⟨10.1016/j.molcatb.2004.01.006⟩. ⟨hal-02680321⟩

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