A tumor necrosis factor decoy receptor homologue is up-regulated in the brook trout (Salvelinus fontinalis) ovary at the completion of ovulation - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Biology of Reproduction Year : 2000

A tumor necrosis factor decoy receptor homologue is up-regulated in the brook trout (Salvelinus fontinalis) ovary at the completion of ovulation

Abstract

An up-regulated cDNA fragment was obtained from differential-display polymerase chain reaction of brook trout ovarian tissue stimulated by phorbol-12-myristate-13-acetate (PMA) and calcium ionophore A23187. Using this cDNA as a probe, a full-length cDNA of 2267 base pairs was obtained by screening a library of PMA/A23187-stimulated ovarian cDNA. The mRNA obtained presumably encodes for a 302-amino acid protein showing similarities with several members of the tumor necrosis factor (TNF) receptor superfamily. The protein contains several cysteine-rich domains characteristic of mammalian TNF receptor members and is most similar to human decoy receptor 3 and osteoprotegerin, two soluble decoy TNF receptors. Consequently, this TNF receptor homologue was tentatively named a trout decoy receptor (TDcR). On Northern blots of ovarian tissue, TDcR hybridized with a 2.2-kilobase transcript that was strongly up-regulated under phorbol ester stimulation. TDcR mRNA was localized in granulosa cells and was detected in the ovary during and after natural ovulation. Its expression was up-regulated at the end of ovulation and progressively down-regulated after 48 h postovulation. Among other trout tissues tested, the transcript was present only in the testis. To our knowledge this is the first description of a member of the TNF receptor family from a lower vertebrate and the first report of a decoy-like TNF receptor in the vertebrate ovary.

Dates and versions

hal-02693463 , version 1 (01-06-2020)

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Julien Bobe, Frederick William Goetz. A tumor necrosis factor decoy receptor homologue is up-regulated in the brook trout (Salvelinus fontinalis) ovary at the completion of ovulation. Biology of Reproduction, 2000, 62, pp.420-426. ⟨10.1095/biolreprod62.2.420⟩. ⟨hal-02693463⟩

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