N2O production, a widespread trait in fungi - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement
Conference Papers Year : 2015

N2O production, a widespread trait in fungi

Laurent Philippot

Abstract

N2O is a powerful greenhouse gas contributing both to global warming and ozone depletion. While fungi have been identified as a putative source of N2O, little is known about their production of this greenhouse gas. Here we investigated the N2O-producing ability of a collection of 207 fungal isolates. Seventy strains producing N2O in pure culture were identified. They were mostly species from the order Hypocreales order-particularly Fusarium oxysporum and Trichoderma spp.-and to a lesser extent species from the orders Eurotiales, Sordariales, and Chaetosphaeriales. The N2O 15N site preference (SP) values of the fungal strains ranged from 15.8% to 36.7% , and we observed a significant taxa effect, with Penicillium strains displaying lower SP values than the other fungal genera. Inoculation of 15 N2O-producing strains into pre-sterilized arable, forest and grassland soils confirmed the ability of the strains to produce N2O in soil with a significant strain-by-soil effect. The copper-containing nitrite reductase gene (nirK) was amplified from 45 N2O-producing strains, and its genetic variability showed a strong congruence with the ITS phylogeny, indicating vertical inheritance of this trait. Taken together, this comprehensive set of findings should enhance our knowledge of fungi as a source of N2O in the environment.

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Dates and versions

hal-02738525 , version 1 (02-06-2020)

Identifiers

  • HAL Id : hal-02738525 , version 1
  • PRODINRA : 373623

Cite

Laurent Philippot. N2O production, a widespread trait in fungi. 7. Colloque AFEM Association Francophone d'Ecologie Microbienne, "Microbiologie et environnement : fondamentaux et applications" , Université de Pau et des Pays de l'Adour (UPPA). FRA., Nov 2015, Anglet, France. ⟨hal-02738525⟩
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