Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Focused ultrasound influence on calcein-loaded thermosensitive stealth liposomes

Abstract : Focused ultrasound (FUS) is a versatile technology for non-invasive thermal therapies in oncology. Indeed, this technology has great potential for local heat-mediated drug delivery from thermosensitive liposomes (TSLs), thus improving therapeutic efficacy and reducing toxicity profiles. In the present study we evaluated the influence of FUS parameters on the release of calcein from TSLs used to model a hydrophilic drug. Quantitative calcein release from TSLs (DPPC/CHOL/DSPE-PEG2000: 90/5/5) and non-thermosensitive liposomes (NTSLs) (DPPC/CHOL/DSPE-PEG2000: 65/30/5) was measured by spectrofluorimetry after both water bath and FUS-induced in vitro heating. The heating of TSLs at 42 degrees C in a water bath resulted in a maximum calcein release of 45%. No additional calcein release was observed at temperatures above 42 degrees C. A similar percentage of calcein release was achieved when TSLs were exposed to 1 MHz sinusoidal waves at peak negative pressure of 1.5 MPa, 40% duty cycle, for 10 min (i.e. above 42 degrees C). No release was detected when NTSLs were heated in a water bath. For both TSLs and NTSLs, the calcein release was increased by more than 10% for acoustic pressures ranging from 1.5 MPa to 2 MPa. This additional release was attributed to the mechanical stress generated by FUS, which was sufficient to disrupt the liposomal membrane. Furthermore, analysis of cryo-TEM images showed a significant decrease in liposome size (14%) induced by the thermal effect, whereas the liposome diameter remained unaffected by the FUS-triggered non-thermal effects.
Type de document :
Article dans une revue
Liste complète des métadonnées

https://www.hal.inserm.fr/inserm-02438261
Déposant : Jean-Michel Escoffre <>
Soumis le : mardi 14 janvier 2020 - 10:39:35
Dernière modification le : mercredi 8 juillet 2020 - 11:44:07

Identifiants

Citation

Anthony Novell, Chantal Al Sabbagh, Jean-Michel Escoffre, Cedric Gaillard, Nicolas Tsapis, et al.. Focused ultrasound influence on calcein-loaded thermosensitive stealth liposomes. International Journal of Hyperthermia, Taylor & Francis, 2015, 31 (4), pp.349-358. ⟨10.3109/02656736.2014.1000393⟩. ⟨inserm-02438261⟩

Partager

Métriques

Consultations de la notice

93