Automatic monitoring devices placed at the entrances of honey bee hives have facilitated the detection of various sublethal effects related to pesticide exposure, such as homing failure and reduced flight activity. These devices have further demonstrated that different neurotoxic pesticide molecules produce similar sublethal impacts on flight activity. The detection of these effects was conducted a posteriori, following the recording of flight activity data. This study introduces a method using an artificial intelligence model, specifically a recurrent neural network, to detect the sublethal effects of pesticides in real-time based on honey bee flight activity. This model was trained on a flight activity dataset comprising 42,092 flight records from 1107 control and 1689 pesticide- exposed bees. The model was able to classify honey bees as healthy or pesticide-exposed based on the number of flights and minutes spent foraging per day. The model was the least accurate (68.46%) when only five days of records per bee were used for training. However, the highest classification accuracy of 99%, a Cohen Kappa of 0.9766, a precision of 0.99, a recall of 0.99, and an F1-score of 0.99 was achieved with the model trained on 25 days of activity data, signifying near-perfect classification ability. These results underscore the highly predictive performance of AI models for toxicovigilance and highlight the potential of our approach for real-time and cost- effective monitoring of risks due to exposure to neurotoxic pesticide in honey bee populations.