Divergent selection for feed efficiency in pigs altered the duodenum transcriptomic response to feed intake and its DNA methylation profiles - Livestock Phenotyping for Sustainable Agro-ecological Systems Access content directly
Journal Articles Physiological Genomics Year : 2024

Divergent selection for feed efficiency in pigs altered the duodenum transcriptomic response to feed intake and its DNA methylation profiles

Katia Feve
Yvon Billon
Julie Demars

Abstract

Feed efficiency is a trait of interest in pigs as it contributes to lowering the ecological and economical costs of pig production. A divergent genetic selection experiment from a Large White pig population was performed for 10 generations, leading to pig lines with relative low- (LRFI) and high- (HRFI) residual feed intake (RFI). Feeding behavior and metabolic differences have been previously reported between the two lines. We hypothesized that part of these differences could be related to differential sensing and absorption of nutrients in the proximal intestine. We investigated the duodenum transcriptome and DNA methylation profiles comparing overnight fasting with ad libitum feeding in LRFI and HRFI pigs (n=24). We identified 1,106 differentially expressed genes between the two lines, notably affecting pathways of the transmembrane transport activity and related to mitosis or chromosome separation. The LRFI line showed a greater transcriptomic response to feed intake than the HRFI line. Feed intake affected genes from both anabolic and catabolic pathways in the pig duodenum, such as rRNA production and autophagy. Several nutrient transporter and tight junction genes were differentially expressed between lines and/or by short term feed intake. We also identified 409 differentially methylated regions in the duodenum mucosa between the two lines, while this epigenetic mark was less affected by feeding. Our findings highlighted that the genetic selection for feed efficiency in pigs changed the transcriptome profiles of the duodenum, and notably its response to feed intake, suggesting key roles for this proximal gut segment in mechanisms underlying feed efficiency.
Fichier principal
Vignette du fichier
Merged_PDF-4.pdf (1.79 Mo) Télécharger le fichier
Origin Files produced by the author(s)

Dates and versions

hal-04528954 , version 1 (29-05-2024)

Identifiers

Cite

Guillaume Devailly, Katia Feve, Safia Saci, Julien Sarry, Sophie Valière, et al.. Divergent selection for feed efficiency in pigs altered the duodenum transcriptomic response to feed intake and its DNA methylation profiles. Physiological Genomics, 2024, ⟨10.1152/physiolgenomics.00123.2023⟩. ⟨hal-04528954⟩
39 View
6 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More