Isopropanol production from carbon dioxide in Cupriavidus necator in a pressurized bioreactor - Archive ouverte HAL Access content directly
Journal Articles New Biotechnology Year : 2020

Isopropanol production from carbon dioxide in Cupriavidus necator in a pressurized bioreactor

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Abstract

A bioreactor was designed to provide high gas mass transfer to reach cell and product titres in the g L-1 level from CO2 for realistic, laboratory scale, engineered autotrophic strain evaluation. The design was based on independent CO2, H-2 and air inputs and the ability to operate at high pressures. The bioreactor configuration and cultivation strategy enabled growth of Cupriavidus necator strains for long periods, to reach over 3 g L-1 dry cell weight. No negative impact of the high pressure was observed on viability of the strains up to more than 4 bar overpressure. The cultivation was then carried out using an engineered isopropanol producing strain; in this case, 3.5 g L-1 isopropanol was obtained from CO2 as the sole carbon source. This is the first reported demonstration of a successful production from engineered bacteria of product in the g L-1 range on CO2 , raising the prospect of future development of CO2-based bioprocesses.
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Dates and versions

hal-02518899 , version 1 (21-07-2022)

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Attribution - NonCommercial - CC BY 4.0

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Lucile Garrigues, Louna Maignien, Eric Lombard, Jasbir Singh, Stéphane Guillouet. Isopropanol production from carbon dioxide in Cupriavidus necator in a pressurized bioreactor. New Biotechnology, 2020, 56, pp.16-20. ⟨10.1016/j.nbt.2019.11.005⟩. ⟨hal-02518899⟩
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