The program of PSDR (for and on the regional development of the regions of Aquitaine and midi-pyrénées) research INGEDICO (Instruments of management and collective devices of conservation and recovery of naturals and renewable ressources) is a pluridisciplinary project associating human and biotechnical sciences. The first results allow us to analyze the territory of limestone hills to implement experimental bio-technological protocols to promote innovating environmental practices. Concretely, this territorial approach of truffle practices makes it possible to study the organization of actors and whether or not they contribute the preservation of these natural resources and landscape with their practices and to propose mobilizable instruments for a concerted management of limestone hills and ecosystems of truffles according to present environmental issues. This article presents us the first results concerning the regional Park of limestone hills in the Quercy (Lot, France).