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Development and validation of a new HPLC-ESI-MS/MS method, and comparison between on-line and off-line SPE for the analysis of glyphosate and AMPA in freshwaters

Abstract : A new C18 column with Ethylene Bridged Hybrid (BEH) particles was used for the determination of 9-fluorenylmethylchloroformate (FMOC) derivatives of glyphosate and its main metabolite aminomethylphosphonic acid (AMPA) by high performance liquid chromatography coupled to electrospray tandem mass spectrometry (HPLCESI-MS/MS). High efficiency and sensitivity were obtained by using high pH mobile phase buffer that consists of triethylamine (TEA) adjusted with acetic acid. The analytical method involves a sample treatment, a derivatization step and an off-line solid-phase extraction (SPE) sample clean-up/enrichement. An alternative on-line SPE method was developped and the performance of the two approaches were compared.
Mots-clés : HPLC
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Conference papers
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https://hal.inrae.fr/hal-02593474
Contributor : Migration Irstea Publications <>
Submitted on : Friday, May 15, 2020 - 5:21:43 PM
Last modification on : Thursday, June 3, 2021 - 5:34:03 PM

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  • HAL Id : hal-02593474, version 1
  • IRSTEA : PUB00029312

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T.T. Nhu Trang, B. Delest, Nicolas Mazzella, François Delmas. Development and validation of a new HPLC-ESI-MS/MS method, and comparison between on-line and off-line SPE for the analysis of glyphosate and AMPA in freshwaters. 6th European Conference Pesticides And Related Organic Micropollutants in the Environment. 12th Symposium on Chemistry and Fate of Modern Pesticides, Sep 2010, Matera, Italy. pp.3. ⟨hal-02593474⟩

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