POCIS (Polar Organic Chemical Integrative Sampler) is a recent integrative sampler made for sampling polar organic micropollutants in water. It is still in development concerning its domain of validity (e.g., molecules sampled, type of water studied, optimal exposure duration) and its performances (molecules sampling rates, repeatability, accuracy of the evaluation of time-weighted average concentrations - TWAC). In order to obtain in situ TWAC, POCIS needs to be calibrated in laboratory for each molecule of interest. In this work, we performed laboratory calibration of POCIS for 5 alkylphenols, 9 hormones, 11 pesticides, 27 pharmaceuticals, 3 phenols and 1 UV filter in a flow-through system. The system is composed of 2 aquaria (50 L) containing spiked tap water (circa 3 µg/L for each studied molecule) and non-spiked POCIS. We present sampling rates for the different studied molecules using our laboratory calibration.