Habitat change was identified by the Millennium Ecosystem Assessment as the main direct driver of biodiversity loss. However, while habitat loss is already implemented in Life Cycle Impact Assessment (LCIA) methods, the additional impact on biodiversity due to habitat fragmentation is not assessed yet. Thus, the goal of this study was to include fragmentation effects from land occupation and transformation at both midpoint and endpoint levels in LCIA. Methods. One promising metric, combining the landscape spatial configuration with species characteristics, is the metapopulation capacity λ, which can be used to rank landscapes in terms of their capacity to support viable populations spatially structured. A methodology to derive worldwide regionalised fragmentation indexes based on λ was used and combined with the Species Fragmented-Area Relationship (SFAR), which relies on λ to assess a species loss due to fragmentation. We adapted both developments to assess fragmentation impacts due to land occupation and transformation at both midpoint and endpoint levels in LCIA. An application to sugarcane production occurring in different geographical areas, more or less sensitive to land fragmentation, was performed. Results and discussion. The comparison to other existing LCIA indicators highlighted its great potential for complementing current assessments through fragmentation effect inclusion. Last, both models were discussed through the evaluation grid used by the UNEP-SETAC land use LCIA working group for biodiversity impact assessment models. Conclusions. Midpoint and endpoint characterisation factors were successfully developed to include the impacts of habitat fragmentation on species in LCIA. For now, they are provided for bird species in all forest ecoregions belonging to the biodiversity hotspots. Further work is required to develop characterisation factors for all taxa and all terrestrial ecoregions.