Yeast Yarrowia lipolytica is a handy tool for efficient production of heterologous proteins. In this work we report the construction of integration vectors for secretion of bacterial phytase by these yeasts. Pantoea sp. 3.5.1 histidine acid phytase encoding gene sequence was codon-optimized and chemically synthetized. Optimized and native phytase gene sequences were cloned into integrative vector pINA1296, containing signal sequence of XPR2 gene. Vectors were multiplied in E.coli DH5α, isolated and linearized for successful integration into Y. lipolytica genome by homologeous recombination in pBR-region. Y. lipolytica strains with integrated bacterial native and optimized phytase genes were obtained.