ATR-FTIR spectroscopy to determine cell wall composition: Application on a large diversity of fruits and vegetables - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Carbohydrate Polymers Year : 2019

ATR-FTIR spectroscopy to determine cell wall composition: Application on a large diversity of fruits and vegetables

Abstract

Infrared spectroscopy coupled with multivariate analyses such as linear regressions was applied to assess the main cell wall components of a huge diversity of fruits and vegetables belonging to 29 plant species. The methodology was tested on the raw freeze-dried powders and on their corresponding AIS (Alcohol Insoluble Solids) dried by solvent exchanges. The most informative spectral region was 1750-1035 cm−1. Excellent predictions (determination coefficient R2≥0.9 and residual predictive deviation RPD≥3.0) were obtained for AIS yields and for arabinose, total glucose, non-cellulosic glucose, total neutral sugars, methanol and starch contents in the AIS samples. The key wavenumbers were: 1740 cm-1 for total neutral sugars; 1075, 1440–1450, 1616 and 1740 cm-1 for pectins; 895, 1035–1041 and 1160-1163 cm-1 for cellulose and 1035–1041 cm-1 for lignin. Limitations of the reference methods to analyze cell wall components (biochemical assays, spectrophotometry, chromatography) affecting the prediction accuracy were also discussed.

Dates and versions

hal-02625630 , version 1 (26-05-2020)

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Maria H.G. Canteri, Catherine M.G.C. Renard, Carine Le Bourvellec, Sylvie Bureau. ATR-FTIR spectroscopy to determine cell wall composition: Application on a large diversity of fruits and vegetables. Carbohydrate Polymers, 2019, 212, pp.186-196. ⟨10.1016/j.carbpol.2019.02.021⟩. ⟨hal-02625630⟩
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