Astin c production by the endophytic fungus cyanodermella asteris in planktonic and immobilized culture conditions - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue Biotechnology Journal Année : 2019

Astin c production by the endophytic fungus cyanodermella asteris in planktonic and immobilized culture conditions

Anthony Arguelles-Arias
Marc Ongena
Philippe Jacques
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Résumé

The fungal endophyte Cyanodermella asteris (C. asteris) has been recently isolated from the medicinal plant Aster tataricus (A. tataricus). This fungus produces astin C, a cyclic pentapeptide with anticancer and anti-inflammatory properties. The production of this secondary metabolite is compared in immobilized and planktonic conditions. For immobilized cultures, a stainless steel packing immersed in the culture broth is used as a support. In these conditions, the fungus exclusively grows on the packing, which provides a considerable advantage for astin C recovery and purification. C. asteris metabolism is different according to the culture conditions in terms of substrate consumption rate, cell growth, and astin C production. Immobilized-cell cultures yield a 30% increase of astin C production, associated with a 39% increase in biomass. The inoculum type as spores rather than hyphae, and a pre-inoculation washing procedure with sodium hydroxide, turns out to be beneficial both for astin C production and fungus development onto the support. Finally, the influence of culture parameters such as pH and medium composition on astin C production is evaluated. With optimized culture conditions, astin C yield is further improved reaching a five times higher final specific yield compared to the value reported with astin C extraction from A. tataricus (0.89 mg g(-1) and 0.16 mg g(-1) respectively).
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Dates et versions

hal-02627915 , version 1 (26-05-2020)

Identifiants

Citer

Antoine Vassaux, Cedric Tarayre, Anthony Arguelles-Arias, Philippe Compère, Frank Delvigne, et al.. Astin c production by the endophytic fungus cyanodermella asteris in planktonic and immobilized culture conditions. Biotechnology Journal, 2019, 14 (8), pp.1-11. ⟨10.1002/biot.201800624⟩. ⟨hal-02627915⟩
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