Pedogenesis is a set of steps which leads to the formation and evolution of soils under pedogenetic factors and processes (e.g., aggregation, weathering, transfer). To describe them quantitatively for a modeling end, constructed Technosols are suitable candidates to be studied, because their initial composition can be controlled. The challenging objective of our work was then to monitor and study nondestructively, visually, and quantitatively the effect of biological agents on the evolution of a constructed Technosol. The Technosol is constructed in three horizons. From bottom to top of the mesocosms, horizons are: (1) gravels, (2) treated industrial soil mixed with paper mill sludge (2/3, 1/3 mass ratio), and (3) green waste compost. Pedogenetic factors are organized according to two modalities each repeated three times: "Plant and Fauna," where six adult earthworms, Lumbricus castaneus, and five seeds of white lupin, Lupinus albus, are inoculated, and a "control" without any plant and macrofauna. Moisture of 60/80 % field capacity is maintained in all treatments throughout a 14-month experiment. Soil evolution is studied by recurrent image acquisition of the soil profile. At the beginning, roots grew preferentially through fissures and cracks at 10 mm center dot day(-1) speed during the first 3 weeks. Then they grew exponentially until reaching a plateau and decreased when plants were at the end of their life cycle. Earthworms prospected the top of the soil first before exploring the deeper horizons preferentially along roots. During their round-trip between the two horizons, earthworms translocated compost. The porosity increased in the first hours of experience and decreased when the system was irrigated. In the Control, porosity continuously decreased while it increased in Fauna-Plant treatment. The evolution of aggregation is root system-dependent. Aggregation was constant in control but significantly increased in Fauna-Plant treatment (about 10 times at 268 days compared with the control). The use of nondestructive observation of soil profiles is therefore an innovative way of monitoring and quantifying the impact of pedogenetic factors on the functioning and evolution of Technosols. Porosity and aggregation increased with time under the influence of biological factors. Constructed Technosols could be used as model soils for studying the dynamics of soil structure. Although their composition is likely to be different from natural soils, the pedogenetic evolution of Technosols is similar to that of natural soils when facing the impact of biological factors.