A tetracycline-inducible expression vector for Streptococcus agalactiae allowing controllable gene expression.
Résumé
Streptococcus agalactiae is the principal cause of neonatal infections; however, for its study, genetic tools are scarce. We have developed pG+off, an expression vector with a tetracycline inducible promoter that allows a tightly-controllable gene expression in S. agalactiae. Using a reporter gene, we have shown that pG+off is characterized by tight repression under non-induced condition.